概述

  • 产品名称Anti-USP5抗体
    参阅全部 USP5 一抗
  • 描述
    兔多克隆抗体to USP5
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 375 and 425 of Human USP5 (CAA62690.1).

  • 阳性对照
    • Human Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma and Testicular Seminoma.
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

应用

Our Abpromise guarantee covers the use of ab84695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • 序列相似性Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • 结构域The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • 翻译后修饰The N-terminus is blocked.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deubiquitinating enzyme 5 antibody
      • Isopeptidase T antibody
      • ISOT 1 antibody
      • ISOT antibody
      • Ubiquitin carboxyl-terminal hydrolase 5 antibody
      • Ubiquitin isopeptidase T antibody
      • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
      • Ubiquitin specific peptidase 5 antibody
      • Ubiquitin specific processing protease 5 antibody
      • Ubiquitin specific protease 5 (isopeptidase T) antibody
      • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
      • Ubiquitin thioesterase 5 antibody
      • Ubiquitin thiolesterase 5 antibody
      • Ubiquitin-specific-processing protease 5 antibody
      • UBP5_HUMAN antibody
      • Usp5 antibody
      see all

    Anti-USP5 antibody 图像

    • ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
    • ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using DAB staining.
    • ab84695, at 1/500 dilution, staining USP5 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using DAB staining.

    Anti-USP5 antibody (ab84695)参考文献

    ab84695 has not yet been referenced specifically in any publications.

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