概述

  • 产品名称Anti-USP5抗体
    参阅全部 USP5 一抗
  • 描述
    兔多克隆抗体to USP5
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) mapped to a region between residue 375 and 425

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab80595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • 序列相似性Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • 结构域The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • 翻译后修饰The N-terminus is blocked.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deubiquitinating enzyme 5 antibody
      • Isopeptidase T antibody
      • ISOT 1 antibody
      • ISOT antibody
      • Ubiquitin carboxyl-terminal hydrolase 5 antibody
      • Ubiquitin isopeptidase T antibody
      • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
      • Ubiquitin specific peptidase 5 antibody
      • Ubiquitin specific processing protease 5 antibody
      • Ubiquitin specific protease 5 (isopeptidase T) antibody
      • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
      • Ubiquitin thioesterase 5 antibody
      • Ubiquitin thiolesterase 5 antibody
      • Ubiquitin-specific-processing protease 5 antibody
      • UBP5_HUMAN antibody
      • Usp5 antibody
      see all

    Anti-USP5 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP5 with ab80595 at 1/1000 (1µg/ml). Detection: DAB.

    Anti-USP5 antibody (ab80595)参考文献

    ab80595 has not yet been referenced specifically in any publications.

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