Anti-USP48抗体(ab72226)

概述

  • 产品名称Anti-USP48抗体
    参阅全部 USP48 一抗
  • 描述
    兔多克隆抗体to USP48
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residues 985 and 1035 of human USP48 (NP_115612.4).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab72226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 3 DUSP domains.
    Contains 1 ubiquitin-like domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAH67261 antibody
    • Deubiquitinating enzyme 48 antibody
    • DKFZp762M1713 antibody
    • EC 3.1.2.15 antibody
    • Kiaa4202 antibody
    • MGC132556 antibody
    • MGC14879 antibody
    • RAP1GA1 antibody
    • Synaptic ubiquitin specific protease antibody
    • synUSP antibody
    • Ubiquitin carboxyl terminal hydrolase 48 antibody
    • Ubiquitin carboxyl-terminal hydrolase 48 antibody
    • Ubiquitin specific peptidase 48 antibody
    • Ubiquitin specific processing protease 48 antibody
    • Ubiquitin specific protease 31 antibody
    • Ubiquitin specific protease 48 antibody
    • Ubiquitin thioesterase 48 antibody
    • Ubiquitin thiolesterase 48 antibody
    • Ubiquitin-specific-processing protease 48 antibody
    • UBP48_HUMAN antibody
    • USP31 antibody
    • USP48 antibody
    see all

Anti-USP48 antibody 图像

  • All lanes : Anti-USP48 antibody (ab72226) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 119 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of USP48 by Western Blot of Immunprecipitate.
    ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.
  • ICC/IF image of ab72226 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-USP48 antibody (ab72226)参考文献

ab72226 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification Egg extracts
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Dec 22 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HaCaT cells)
Specification HaCaT cells
Treatment USP48 siRNA (300pM per 10 cm dish) for 48 hours or control treated
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Oct 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hela Cells)
Loading amount 50 µg
Specification Hela Cells
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"