概述

  • 产品名称Anti-USP47抗体
  • 描述
    兔多克隆抗体to USP47
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)

  • 阳性对照
    • HeLa whole cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性USP47 belongs to the peptidase C19 family and may be catalytically inactive although it seems to have kept all necessary active site residues. It is expressed in skeletal muscle, heart and testis and there are 3 named isoforms produced by alternative splicing.
    • 数据库链接
    • 别名
      • Deubiquitinating enzyme 47 antibody
      • TRF (TATA bidning protein-related factor) proximal homolog antibody
      • TRFP antibody
      • Ubiquitin carboxyl terminal hydrolase 47 antibody
      • ubiquitin specific peptidase 47 antibody
      • Ubiquitin specific processing protease 47 antibody
      • Ubiquitin thioesterase 47 antibody
      • USP 47 antibody
      see all

    Anti-USP47 antibody 图像

    • All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 158 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds
    • Detection of USP47 by Western Blot of Immunprecipitate.
      ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    Anti-USP47 antibody (ab72143)参考文献

    This product has been referenced in:
    • Sako-Kubota K  et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). Read more (PubMed: 25253721) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"