Recombinant fragment corresponding to amino acids 454-580 of Human USP42 (Q9H9J4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Deubiquitinating enzyme which may play an important role during spermatogenesis.
Belongs to the peptidase C19 family.
Information by UniProt
- Deubiquitinating enzyme 42 antibody
- FLJ12697 antibody
- Ubiquitin carboxyl-terminal hydrolase 42 antibody
Immunocytochemistry/ Immunofluorescence - Anti-USP42 antibody (ab121254)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121254 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP42 antibody (ab121254)
Immunohistochemical staining of parffin embedded Human kidney, using 1/60 ab121254, shows strong nuclear cytoplasmic positivity in cells in tubules and cells in glomeruli.
has not yet been referenced specifically in any publications.
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