Anti-USP40抗体(ab121234)

概述

  • 产品名称Anti-USP40抗体
  • 描述
    兔多克隆抗体to USP40
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 755-900 of Human USP40.

  • 阳性对照
    • Human tonsil tissue

性能

应用

Our Abpromise guarantee covers the use of ab121234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use Buffer at pH 6 for antigen retrieval.

靶标

Anti-USP40 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles and focal adhesions. Recommended concentration of ab121234 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121234, at 1/75 dilution, staining USP40 in Human tonsil tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

Anti-USP40 antibody (ab121234)参考文献

ab121234 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - other (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"