All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa Observed band size: 117 kDa Additional bands at: 31 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - USP38 antibody (ab72244)
Immunoprecipitation/ Western Blot of USP38.
Lane 1: ab72244 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72244 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.