Anti-USP37抗体(ab72199)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to USP37
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 930-979 of Human USP37 (NP_065986.1)

  • 阳性对照
    • HeLa whole cell lysate 239T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab72199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 组织特异性
    Expressed in brain and prostate.
  • 序列相似性
    Belongs to the peptidase C19 family.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 3 antibody
    • Deubiquitinating enzyme 37 antibody
    • EC 3.1.2.15 antibody
    • KIAA1594 antibody
    • MGC117261 antibody
    • OTTHUMP00000164146 antibody
    • tmp_locus_50 antibody
    • Ubiquitin carboxyl-terminal hydrolase 37 antibody
    • Ubiquitin specific peptidase 37 antibody
    • Ubiquitin specific processing protease 37 antibody
    • Ubiquitin specific protease 37 antibody
    • Ubiquitin thioesterase 37 antibody
    • Ubiquitin thiolesterase 37 antibody
    • Ubiquitin-specific-processing protease 37 antibody
    • UBP37_HUMAN antibody
    • USP37 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling USP37 with ab72199 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-USP37 antibody (ab72199) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 110 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95-117 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab72199 at 3µg/mg lysate. 20% of the immunoprecipitate was used for Western blotting and detected using ab72199 at 1µg/ml. Bands were exposed by chemiluminescence with an exposure time of 10 seconds.

文献

ab72199 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Sample
Human Cell lysate - whole cell (Osteoblast)
Specification
Osteoblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carlos le Sage

Verified customer

提交于 Aug 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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