The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 130, 160 kDa (predicted molecular weight: 123 kDa).
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
功能May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP36 with ab72243 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - USP36 antibody (ab72243)
All lanes : Anti-USP36 antibody (ab72243) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of USP36.
Lane 1: ab72243 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72243 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 minutes.
Anti-USP36 antibody (ab72243)参考文献
has not yet been referenced specifically in any publications.