概述

  • 产品名称Anti-USP36抗体
    参阅全部 USP36 一抗
  • 描述
    兔多克隆抗体to USP36
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide from a region between residue 1070 and the C-terminus (residue 1121) of human USP36, (NP_079366.2).

  • 阳性对照
    • Whole cell lysate from Hela cells.

性能

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应用

Our Abpromise guarantee covers the use of ab72243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 130, 160 kDa (predicted molecular weight: 123 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).
  • 组织特异性Broadly expressed.
  • 序列相似性Belongs to the peptidase C19 family.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 1 antibody
    • Deubiquitinating enzyme 36 antibody
    • DUB1 antibody
    • FLJ12851 antibody
    • KIAA1453 antibody
    • Ubiquitin carboxyl-terminal hydrolase 36 antibody
    • Ubiquitin specific peptidase 36 antibody
    • Ubiquitin specific processing protease 36 antibody
    • Ubiquitin thioesterase 36 antibody
    • Ubiquitin thiolesterase 36 antibody
    • Ubiquitin-specific-processing protease 36 antibody
    • UBP36_HUMAN antibody
    • Usp36 antibody
    see all

Anti-USP36 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP36 with ab72243 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-USP36 antibody (ab72243) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 123 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa. We are unsure as to the identity of these extra bands.
    Various other bands are detected.
  • Immunoprecipitation/ Western Blot of USP36.
    Lane 1: ab72243 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72243 at 1µg/ml for subsequent WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 3 minutes.

Anti-USP36 antibody (ab72243)参考文献

ab72243 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (HEK293T cells)
Specification HEK293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"