Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residues 967 and 1017 of human USP35 (CAE51937.1).
HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab86791 was affinity purified using an epitope specific to USP35 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 113 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed in testis, pancreas and skeletal muscle.
Belongs to the peptidase C19 family.
Information by UniProt
Deubiquitinating enzyme 35 antibody
Western blot - Anti-USP35 antibody (ab86791)
All lanes : Anti-USP35 antibody (ab86791) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-USP35 antibody (ab86791)
Detection of USP35 by Western Blot of Immunprecipitate.
ab86791 at 1 µg/ml staining USP35 in HeLa whole cell lysate immunoprecipitated using ab86791 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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