概述

  • 产品名称Anti-USP30抗体
    参阅全部 USP30 一抗
  • 描述
    兔多克隆抗体to USP30
  • 特异性ab74368 antibody detects endogenous levels of total USP30 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human USP30.

  • 阳性对照
    • HeLa cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab74368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/10000.

靶标

  • 功能May participate in the maintenance of mitochondrial morphology.
  • 组织特异性Expressed in skeletal muscle, pancreas, liver and kidney.
  • 序列相似性Belongs to the peptidase C19 family.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 30 antibody
    • FLJ40511 antibody
    • Ub-specific protease 30 antibody
    • Ubiquitin carboxyl terminal hydrolase 30 antibody
    • Ubiquitin carboxyl-terminal hydrolase 30 antibody
    • Ubiquitin specific peptidase 30 antibody
    • Ubiquitin specific processing protease 30 antibody
    • Ubiquitin thioesterase 30 antibody
    • Ubiquitin thiolesterase 30 antibody
    • Ubiquitin-specific-processing protease 30 antibody
    • UBP30_HUMAN antibody
    • usp30 antibody
    see all

Anti-USP30 antibody 图像

  • All lanes : Anti-USP30 antibody (ab74368) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 26 kDa,33 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab74368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74368, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-USP30 antibody (ab74368)参考文献

ab74368 has not yet been referenced specifically in any publications.

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