The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 2 - 5 µg/ml.
应用说明Is unsuitable for WB.
功能Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
组织特异性Expressed in all tissues examined, with strongest expression in pancreas.
序列相似性Belongs to the peptidase C19 family. USP3 subfamily. Contains 1 UBP-type zinc finger.
结构域Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.
细胞定位Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.