概述

  • 产品名称Anti-USP28抗体
    参阅全部 USP28 一抗
  • 描述
    兔多克隆抗体to USP28
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of human USP28 (NP_065937.1)

  • 阳性对照
    • Human colon and stomach adenocarcinoma tissues.

性能

应用

Our Abpromise guarantee covers the use of ab84514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.

靶标

  • 功能Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
  • 序列相似性Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • 翻译后修饰Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

Anti-USP28 antibody 图像

  • ab84514 at 1/250 dilution staining USP28 in human colon adenocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab84514 at 1/250 dilution staining USP28 in human stomach adenocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-USP28 antibody (ab84514)参考文献

ab84514 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84514.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"