概述

  • 产品名称Anti-USP28抗体
    参阅全部 USP28 一抗
  • 描述
    兔多克隆抗体to USP28
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 50 and 100 of human USP28 (NP_065937.1).

  • 阳性对照
    • Whole cell lysates from 293T and HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 140, 100 kDa (predicted molecular weight: 122 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
  • 序列相似性Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • 翻译后修饰Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

Anti-USP28 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP28 with ab70893 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-USP28 antibody (ab70893) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 122 kDa
    Observed band size : 110,140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of Human USP28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70893 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Note: bands between 55 and 71 kDa represent the IgG chains.

Anti-USP28 antibody (ab70893)参考文献

ab70893 has not yet been referenced specifically in any publications.

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