Anti-USP28抗体(ab188240)
Key features and details
- Rabbit polyclonal to USP28
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-USP28抗体
参阅全部 USP28 一抗 -
描述
兔多克隆抗体to USP28 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human USP28 aa 1-400.
Database link: Q96RU2 -
阳性对照
- U2OS, NIH 3T3 and INS-1 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188240于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 122 kDa.
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ICC/IF |
1/2000.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
1/2000. Predicted molecular weight: 122 kDa. |
ICC/IF
1/2000. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. -
序列相似性
Belongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
翻译后修饰
Degradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. -
细胞定位
Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 57646 Human
- Entrez Gene: 235323 Mouse
- Entrez Gene: 315639 Rat
- Omim: 610748 Human
- SwissProt: Q96RU2 Human
- SwissProt: Q5I043 Mouse
- SwissProt: D3ZJ96 Rat
- Unigene: 503891 Human
see all -
别名
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
see all
图片
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All lanes : Anti-USP28 antibody (ab188240) at 1/2000 dilution
Lane 1 : Lysate from U2OS cells transfected with a control si
Lane 2 : Lysate from U2OS cells transfected with a USP28 si
Lane 3 : NIH 3T3 cell lysate
Lane 4 : INS-1 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 122 kDa -
Western blot analysis (using ab188240) of immunoprecipitates from U2OS cell extracts
Lane 1: Input
Lane 2: immunoprecipitate with control serum+protein A sepharose.
Lane 3: immunoprecipitate with ab188240 + protein A sepharose.
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Immunofluorescent analysis of 3% PFA-fixed U2OS cells transfected with either a control si (left) or USP28 si (right) labeling USP28 with ab188240 at 1/2000 dilution.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab188240 被引用在 1 文献中.
- Chan WC et al. Accelerating inhibitor discovery for deubiquitinating enzymes. Nat Commun 14:686 (2023). WB ; Human . PubMed: 36754960