概述

  • 产品名称Anti-USP25抗体
    参阅全部 USP25 一抗
  • 描述
    兔多克隆抗体to USP25
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (10-30 aa in length) conjugated to KLH, corresponding to N terminal of USP25 and derived from within residues 173-188 (Human)

  • 阳性对照
    • USP25-transfected HeLa cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab38500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 126 kDa.
ELISA 1/1000.

靶标

  • 功能Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • 组织特异性Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • 翻译后修饰Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • 细胞定位Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 25 antibody
    • Ubiquitin carboxyl-terminal hydrolase 25 antibody
    • Ubiquitin specific peptidase 25 antibody
    • ubiquitin specific protease 25 antibody
    • ubiquitin specific protease USP25 antibody
    • Ubiquitin thiolesterase 25 antibody
    • Ubiquitin-specific-processing protease 25 antibody
    • UBP25_HUMAN antibody
    • USP on chromosome 21 antibody
    • USP21 antibody
    • USP25 antibody
    see all

Anti-USP25 antibody 图像

  • Anti-USP25 antibody (ab38500) + USP25-transfected HeLa cell lysate.

    Predicted band size : 126 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

Anti-USP25 antibody (ab38500)参考文献

This product has been referenced in:
  • Kim S  et al. Vaccinia-Related Kinase 2 Controls the Stability of the Eukaryotic Chaperonin TRiC/CCT by Inhibiting the Deubiquitinating Enzyme USP25. Mol Cell Biol 35:1754-62 (2015). Read more (PubMed: 25755282) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab38500.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"