Anti-USP24抗体(ab72241)

概述

  • 产品名称Anti-USP24抗体
    参阅全部 USP24 一抗
  • 描述
    兔多克隆抗体to USP24
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide from a region between residue 2410 and the C-terminus (residue 2460) of human USP24 (XP_371254.5)

  • 阳性对照
    • Whole cell lysate from Hela and 293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2,000 - 1/10,000. Detects bands of approximately 71 kDa and 250 kDa (predicted molecular weight: 211 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-USP24 antibody 图像

    • All lanes : Anti-USP24 antibody (ab72241) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 211 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of USP24.
      Lane 1: ab72241 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72241 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-USP24 antibody (ab72241)参考文献

    ab72241 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vi...

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    Thank you for contacting Abcam regarding our USP24 antibodies.


    Regarding the difference in molecular weight of the bands recognized by these antibodies:


    ab72241 - The WB image on our datasheet is representative of endogenous...

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