Anti-USP21抗体(ab38864)

Abreviews (1)Q&A (3)

概述

  • 产品名称
    Anti-USP21抗体
    参阅全部 USP21 一抗
  • 描述
    兔多克隆抗体to USP21
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH selected from the C-terminal region of human USP21.

  • 阳性对照
    • Human breast carcinoma tissues.

性能

应用

Our Abpromise guarantee covers the use of ab38864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.
  • 组织特异性
    Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.
  • 序列相似性
    Belongs to the peptidase C19 family. USP21 subfamily.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Deubiquitinating enzyme 21 antibody
      • deubiquitinating enzyme 23 antibody
      • EC 3.1.2.15 antibody
      • ESTM28 antibody
      • MGC3394 antibody
      • NEDD8-specific protease antibody
      • Ubiquitin carboxyl terminal hydrolase 21 antibody
      • ubiquitin carboxyl terminal hydrolase 23 antibody
      • Ubiquitin carboxyl-terminal hydrolase 21 antibody
      • ubiquitin specific peptidase 21 antibody
      • Ubiquitin specific processing protease 21 antibody
      • ubiquitin specific protease 16 antibody
      • ubiquitin specific protease 21 antibody
      • ubiquitin specific protease 23 antibody
      • Ubiquitin thioesterase 21 antibody
      • Ubiquitin thiolesterase 21 antibody
      • ubiquitin thiolesterase 23 antibody
      • ubiquitin-specific protease 23, formerly antibody
      • Ubiquitin-specific-processing protease 21 antibody
      • UBP21_HUMAN antibody
      • USP16 antibody
      • Usp21 antibody
      • USP23 antibody
      • USP23, formerly antibody
      • W53272 antibody
      see all

    图片

    • Western blot - Anti-USP21 antibody (ab38864)
      Western blot - Anti-USP21 antibody (ab38864)
      Anti-USP21 antibody (ab38864) + T47D cell lysate

      Predicted band size: 63 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP21 antibody (ab38864)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP21 antibody (ab38864)
      Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38864, followed by AEC staining.

    文献

    ab38864 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab38864？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    4 reviews or Q&A

    10
    Votes: Highest First

    Sorry for the late reply to this.

    Thanks for the understanding - we would like a refund for the antibody.

    Thank you for contacting us.

    Your credit note ID for the refundis xxx.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note m...

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    1. I see bands everywhere from around 250 kDa all the way to 17 kDa. there is a band around the 55 kDa mark (the only strong band in that area) but it does not alter on transfection with overexpression plasmid or with siRNA against USP21.

    2....

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    Thank you for your reply and for the additional information that will help us to reevaluate this antibody.

    Yes, I'd be happy to compensate you with eithera credit (can be used on future Abcam purchase) or a refund (money back into your accoun...

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    I recently bought the USP21 antibody ab38864. I have tested it, using the protocol on the datasheet, on HEK293T cells transiently transfected with a plasmid expressing USP21 and there is very little difference in any of the many bands that the antibody...

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    Thank you for your email. I am sorry to hear that this antibody is not providing satisfactory results.

    Could you please provide the following additional details whichwill enable us to investigate this case and will provide us with vital info...

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    Western blot abreview for Anti-USP21 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    0.034 µg
    Specification
    Catalytic domain of USP21
    Gel Running Conditions
    Non-reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Human Purified protein (Catalytic domain of USP21)
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    Username

    Mr. Reggy Ekkebus

    Verified customer

    提交于 Jul 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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