Anti-USP20抗体(ab72225)
Key features and details
- Rabbit polyclonal to USP20
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-USP20抗体
参阅全部 USP20 一抗 -
描述
兔多克隆抗体to USP20 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Chicken, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human USP20.
Database link: Q9Y2K6 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
序列相似性
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
Contains 2 DUSP domains.
Contains 1 UBP-type zinc finger. -
结构域
The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue. -
翻译后修饰
Ubiquitinated via a VHL-dependent pathway for proteasomal degradation. -
细胞定位
Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear. - Information by UniProt
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数据库链接
- Entrez Gene: 10868 Human
- SwissProt: Q9Y2K6 Human
- SwissProt: Q5R5Z6 Orangutan
- Unigene: 5452 Human
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别名
- Deubiquitinating enzyme 20 antibody
- hVDU2 antibody
- KIAA1003 antibody
see all
图片
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All lanes : Anti-USP20 antibody (ab72225) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of USP20 by Western Blot of Immunprecipitate.
ab72225 at 1µg/ml staining USP20 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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Datasheet download
文献 (0)
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