概述

  • 产品名称Anti-USP2抗体
    参阅全部 USP2 一抗
  • 描述
    兔多克隆抗体to USP2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human USP2.

    (Peptide available as ab66555.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue Lysates: Pancreas, Prostate, Prostate Tumor

性能

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应用

Our Abpromise guarantee covers the use of ab66556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human USP2 peptide (ab66555).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells.
  • 组织特异性Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level).
  • 序列相似性Belongs to the peptidase C19 family. USP2 subfamily.
  • 结构域The different N-terminus extensions of isoform 1 and isoform 4 determine their respective subcellular localization and differentiel effect on myoblast fusion and accumulation of muscle-specific proteins. The different N-terminus extensions of isoform 1 and isoform 4 are not essential for their catalytic activity.
  • 细胞定位Nucleus and Cytoplasm. Cytoplasm > perinuclear region. Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 41 kDa ubiquitin specific protease antibody
    • 41 kDa ubiquitin-specific protease antibody
    • Deubiquitinating enzyme 2 antibody
    • Ubiquitin carboxyl terminal hydrolase 2 antibody
    • Ubiquitin carboxyl-terminal hydrolase 2 antibody
    • ubiquitin specific peptidase 2 antibody
    • Ubiquitin specific processing protease 2 antibody
    • Ubiquitin specific protease 12 antibody
    • Ubiquitin specific protease 9 antibody
    • Ubiquitin specific protease, 41kD antibody
    • Ubiquitin thioesterase 2 antibody
    • Ubiquitin thiolesterase 2 antibody
    • Ubiquitin-specific-processing protease 2 antibody
    • Ubiquitin-specific-processing protease testis antibody
    • UBP-t antibody
    • UBP2_HUMAN antibody
    • UBP41 antibody
    • USP2 antibody
    • USP9 antibody
    see all

Anti-USP2 antibody 图像

  • All lanes : Anti-USP2 antibody (ab66556) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Human prostate tissue lysate - total protein (ab30304)
    Lane 3 : Prostate Tumor Lysate Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 30 kDa,56 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66556 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66556, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 5µg/ml.

Anti-USP2 antibody (ab66556)参考文献

ab66556 has not yet been referenced specifically in any publications.

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