概述
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产品名称Anti-USP15抗体- Carboxyterminal end
参阅全部 USP15 一抗 -
描述兔多克隆抗体to USP15 - Carboxyterminal end
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Human
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免疫原
A KLH conjugated synthetic peptide selected from the C-terminal region of human USP15.
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阳性对照
- Transfected/ non-transfected 293 cell lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Protein G purified
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纯化说明This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab71242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects bands of approximately 65, 75 and 112 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100. Detects bands of approximately 65, 75 and 112 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
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组织特异性Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
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序列相似性Belongs to the peptidase C19 family.
Contains 1 DUSP domain. -
翻译后修饰Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome. - Information by UniProt
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数据库链接
- Entrez Gene: 9958 Human
- Omim: 604731 Human
- SwissProt: Q9Y4E8 Human
- Unigene: 434951 Human
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别名
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
see all
Anti-USP15 antibody - Carboxyterminal end 图像
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Western blot - USP15 antibody - Carboxyterminal end (ab71242)All lanes : Anti-USP15 antibody - Carboxyterminal end (ab71242) at 1/50 dilution
Lane 1 : Non-transfected 293 cell lysates
Lane 2 : USP15 transfected 293 cell lysates
Lysates/proteins at 2 µg per lane.
Predicted band size : 112 kDa
Observed band size : 112 kDa
Additional bands at : 65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Anti-USP15 antibody - Carboxyterminal end (ab71242)参考文献
ab71242 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"