概述

  • 产品名称Anti-USP15抗体
    参阅全部 USP15 一抗
  • 描述
    兔多克隆抗体to USP15
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    A peptide from the region between residue 960 and the C-terminus of human Ubiquitin Specific Peptidase 15 using the numbering given in entry AAD41086.1 (GeneID9958).

性能

应用

Our Abpromise guarantee covers the use of ab71713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 112 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
  • 组织特异性Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 DUSP domain.
  • 翻译后修饰Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
    Ubiquitinated, leading to degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 15 antibody
    • Deubiquitinating enzyme antibody
    • EC 3 1 2 15 antibody
    • KIAA0529 antibody
    • MGC131982 antibody
    • MGC149838 antibody
    • MGC74854 antibody
    • Ubiquitin Carboxy terminal Hydrolase 15 antibody
    • Ubiquitin carboxyl-terminal hydrolase 15 antibody
    • Ubiquitin specific peptidase 15 antibody
    • Ubiquitin specific processing protease 15 antibody
    • Ubiquitin Specific Protease 15 antibody
    • Ubiquitin thioesterase 15 antibody
    • Ubiquitin thiolesterase 15 antibody
    • Ubiquitin-specific-processing protease 15 antibody
    • UBP 15 antibody
    • UBP15 antibody
    • UBP15_HUMAN antibody
    • Unph 2 antibody
    • UNPH 4 antibody
    • Unph-2 antibody
    • Unph2 antibody
    • Unph4 antibody
    • USP 15 antibody
    • Usp15 antibody
    see all

Anti-USP15 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling USP15 with ab71713 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-USP15 antibody (ab71713) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 112 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • All lanes : Anti-USP15 antibody (ab71713) at 1 µg/ml

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26.WT whole cell lysate
    Lane 5 : C6 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 112 kDa


    Exposure time : 3 minutes
  • USP15 antibody (ab71713) recognizing human USP15 in HeLa whole cell lysate (1 mg, 20% of IP loaded).
    Lane 1: USP15 antibody (ab71713) blotted at 1.0 µg/ml and used for IP at 3 µg/mg lysate.
    Lane 2: Control IgG
    Detection by chemiluminescence with exposure time of 3 minutes.

Anti-USP15 antibody (ab71713)参考文献

ab71713 has not yet been referenced specifically in any publications.

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