概述

  • 产品名称Anti-USP15抗体
    参阅全部 USP15 一抗
  • 描述
    小鼠单克隆抗体to USP15
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-236 of Human USP15

性能

应用

Our Abpromise guarantee covers the use of ab56900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
  • 组织特异性Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 DUSP domain.
  • 翻译后修饰Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
    Ubiquitinated, leading to degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 15 antibody
    • Deubiquitinating enzyme antibody
    • EC 3 1 2 15 antibody
    • KIAA0529 antibody
    • MGC131982 antibody
    • MGC149838 antibody
    • MGC74854 antibody
    • Ubiquitin Carboxy terminal Hydrolase 15 antibody
    • Ubiquitin carboxyl-terminal hydrolase 15 antibody
    • Ubiquitin specific peptidase 15 antibody
    • Ubiquitin specific processing protease 15 antibody
    • Ubiquitin Specific Protease 15 antibody
    • Ubiquitin thioesterase 15 antibody
    • Ubiquitin thiolesterase 15 antibody
    • Ubiquitin-specific-processing protease 15 antibody
    • UBP 15 antibody
    • UBP15 antibody
    • UBP15_HUMAN antibody
    • Unph 2 antibody
    • UNPH 4 antibody
    • Unph-2 antibody
    • Unph2 antibody
    • Unph4 antibody
    • USP 15 antibody
    • Usp15 antibody
    see all

Anti-USP15 antibody 图像

  • Overlay histogram showing HeLa cells stained with ab56900 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56900, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • USP15 antibody (ab56900) used in immunofluorescence at 10ug/ml on HeLa cells.
  • USP15 antibody (ab56900) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

Anti-USP15 antibody (ab56900)参考文献

This product has been referenced in:
  • Pauli EK  et al. The ubiquitin-specific protease USP15 promotes RIG-I-mediated antiviral signaling by deubiquitylating TRIM25. Sci Signal 7:ra3 (2014). Read more (PubMed: 24399297) »
  • Long L  et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem 289:8916-30 (2014). WB . Read more (PubMed: 24526689) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab56900.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"