概述

  • 产品名称
  • 描述
    山羊多克隆抗体to USP15
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human USP15 aa 970-981 (C terminal).
    Sequence:

    NDIENENCMHTN


    Database link: Q9Y4E8

  • 常规说明
    Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.

性能

应用

Our Abpromise guarantee covers the use of ab4850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IHC-P Use a concentration of 2.5 - 3.8 µg/ml.

Perform heat mediated antigen retrieval with citrate buffer (pH6).

靶标

  • 功能
    Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
  • 组织特异性
    Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
  • 序列相似性
    Belongs to the peptidase C19 family.
    Contains 1 DUSP domain.
  • 翻译后修饰
    Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
    Ubiquitinated, leading to degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 15 antibody
    • Deubiquitinating enzyme antibody
    • EC 3 1 2 15 antibody
    • KIAA0529 antibody
    • MGC131982 antibody
    • MGC149838 antibody
    • MGC74854 antibody
    • Ubiquitin Carboxy terminal Hydrolase 15 antibody
    • Ubiquitin carboxyl-terminal hydrolase 15 antibody
    • Ubiquitin specific peptidase 15 antibody
    • Ubiquitin specific processing protease 15 antibody
    • Ubiquitin Specific Protease 15 antibody
    • Ubiquitin thioesterase 15 antibody
    • Ubiquitin thiolesterase 15 antibody
    • Ubiquitin-specific-processing protease 15 antibody
    • UBP 15 antibody
    • UBP15 antibody
    • UBP15_HUMAN antibody
    • Unph 2 antibody
    • UNPH 4 antibody
    • Unph-2 antibody
    • Unph2 antibody
    • Unph4 antibody
    • USP 15 antibody
    • Usp15 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at  2.5-3.8µg/ml.   The image shows patterned intracellular staining in hepatocytes.

    Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

     

  • All lanes : Anti-USP15 antibody (ab4850) at 1/1000 dilution

    Lane 1 : 20µg mouse brain whole cell lysate
    Lane 2 : 20µg mouse brain whole cell lysate
    Lane 3 : 20µg mouse brain whole cell lysate

    Secondary
    Donkey anti-goat conjugated to HRP

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab4850 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (6.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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提交于 Feb 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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