概述

  • 产品名称Anti-USP15抗体
    参阅全部 USP15 一抗
  • 描述
    山羊多克隆抗体to USP15
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human USP15 aa 970-981 (C terminal).
    Sequence:

    NDIENENCMHTN


    Database link: Q9Y4E8

  • 常规说明Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.

性能

应用

Our Abpromise guarantee covers the use of ab4850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IHC-P Use a concentration of 2.5 - 3.8 µg/ml.

Perform heat mediated antigen retrieval with citrate buffer (pH6).

靶标

  • 功能Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
  • 组织特异性Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 DUSP domain.
  • 翻译后修饰Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
    Ubiquitinated, leading to degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 15 antibody
    • Deubiquitinating enzyme antibody
    • EC 3 1 2 15 antibody
    • KIAA0529 antibody
    • MGC131982 antibody
    • MGC149838 antibody
    • MGC74854 antibody
    • Ubiquitin Carboxy terminal Hydrolase 15 antibody
    • Ubiquitin carboxyl-terminal hydrolase 15 antibody
    • Ubiquitin specific peptidase 15 antibody
    • Ubiquitin specific processing protease 15 antibody
    • Ubiquitin Specific Protease 15 antibody
    • Ubiquitin thioesterase 15 antibody
    • Ubiquitin thiolesterase 15 antibody
    • Ubiquitin-specific-processing protease 15 antibody
    • UBP 15 antibody
    • UBP15 antibody
    • UBP15_HUMAN antibody
    • Unph 2 antibody
    • UNPH 4 antibody
    • Unph-2 antibody
    • Unph2 antibody
    • Unph4 antibody
    • USP 15 antibody
    • Usp15 antibody
    see all

Anti-USP15 antibody 图像

  • Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at  2.5-3.8µg/ml.   The image shows patterned intracellular staining in hepatocytes.

    Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

     

  • All lanes : Anti-USP15 antibody (ab4850) at 1/1000 dilution

    Lane 1 : 20µg mouse brain whole cell lysate
    Lane 2 : 20µg mouse brain whole cell lysate
    Lane 3 : 20µg mouse brain whole cell lysate

    Secondary
    Donkey anti-goat conjugated to HRP

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-USP15 antibody (ab4850)参考文献

ab4850 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (6.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Feb 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"