概述

  • 产品名称Anti-USP13抗体
    参阅全部 USP13 一抗
  • 描述
    兔多克隆抗体to USP13
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 750-800 of Human USP13 (NP_003931.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab99423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性Highly expressed in ovary and testes.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 13 antibody
    • Isopeptidase T 3 antibody
    • Isopeptidase T-3 antibody
    • Isopeptidase T3 antibody
    • ISOT 3 antibody
    • ISOT-3 antibody
    • ISOT3 antibody
    • Ubiquitin carboxyl terminal hydrolase 13 antibody
    • Ubiquitin carboxyl-terminal hydrolase 13 antibody
    • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
    • Ubiquitin specific peptidase 13 antibody
    • Ubiquitin specific processing protease 13 antibody
    • Ubiquitin specific protease 13 antibody
    • Ubiquitin thioesterase 13 antibody
    • Ubiquitin thiolesterase 13 antibody
    • Ubiquitin-specific-processing protease 13 antibody
    • UBP13 antibody
    • UBP13_HUMAN antibody
    • USP 13 antibody
    • USP13 antibody
    see all

Anti-USP13 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP13 with ab99423 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-USP13 antibody (ab99423) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa


    Exposure time : 30 seconds
  • ab99423 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
    For immunoprecipitation, ab99423 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99421, a rabbit anti-USP13 antibody which recognizes an upstream epitope.
    Lane 2; IP using ab99423.
    Lane 3; IP using control IgG.

Anti-USP13 antibody (ab99423)参考文献

ab99423 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99423.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"