Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP13 with ab99423 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - USP13 antibody (ab99423)
All lanes : Anti-USP13 antibody (ab99423) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 97 kDa
Exposure time : 30 seconds
Immunoprecipitation - USP13 antibody (ab99423)
ab99423 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
For immunoprecipitation, ab99423 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99421, a rabbit anti-USP13 antibody which recognizes an upstream epitope.
Lane 2; IP using ab99423.
Lane 3; IP using control IgG.
Anti-USP13 antibody (ab99423)参考文献
has not yet been referenced specifically in any publications.