概述

  • 产品名称Anti-USP13抗体
    参阅全部 USP13 一抗
  • 描述
    兔多克隆抗体to USP13
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human USP13 (NP_003931.2).

  • 阳性对照
    • HeLa and 293T whole cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

性能

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应用

Our Abpromise guarantee covers the use of ab99421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 组织特异性Highly expressed in ovary and testes.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 13 antibody
    • Isopeptidase T 3 antibody
    • Isopeptidase T-3 antibody
    • Isopeptidase T3 antibody
    • ISOT 3 antibody
    • ISOT-3 antibody
    • ISOT3 antibody
    • Ubiquitin carboxyl terminal hydrolase 13 antibody
    • Ubiquitin carboxyl-terminal hydrolase 13 antibody
    • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
    • Ubiquitin specific peptidase 13 antibody
    • Ubiquitin specific processing protease 13 antibody
    • Ubiquitin specific protease 13 antibody
    • Ubiquitin thioesterase 13 antibody
    • Ubiquitin thiolesterase 13 antibody
    • Ubiquitin-specific-processing protease 13 antibody
    • UBP13 antibody
    • UBP13_HUMAN antibody
    • USP 13 antibody
    • USP13 antibody
    see all

Anti-USP13 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma tissues labelling USP13 with ab99421 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab99421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99421 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-USP13 antibody (ab99421) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa


    Exposure time : 3 minutes
  • ab99421 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
    For immunoprecipitation, ab99421 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99421
    Lane 2; IP using ab99423, a rabbit anti-USP13 antibody which recognizes a downstream epitope.
    Lane 3; IP using control IgG.

Anti-USP13 antibody (ab99421)参考文献

This product has been referenced in:
  • Ouyang H  et al. Protein Aggregates Are Recruited to Aggresome by Histone Deacetylase 6 via Unanchored Ubiquitin C Termini. J Biol Chem 287:2317-27 (2012). Read more (PubMed: 22069321) »

See 1 Publication for this product

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