Anti-USP12抗体(ab89870)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to USP12
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (TRLRKENELF DNYMQQDAHE FLNYLLNTIA DILQEERKQE KQNGRLPNGN) of Human USP12 (NP_872294).

  • 阳性对照
    • Human Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab89870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
  • 序列相似性
    Belongs to the peptidase C19 family. USP12/USP46 subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 12 antibody
    • OTTHUMP00000042337 antibody
    • UBH 1 antibody
    • UBH1 antibody
    • Ubiquitin carboxyl terminal hydrolase 12 antibody
    • Ubiquitin carboxyl-terminal hydrolase 12 antibody
    • Ubiquitin hydrolyzing enzyme 1 antibody
    • Ubiquitin specific peptidase 12 antibody
    • Ubiquitin specific processing protease 12 antibody
    • Ubiquitin specific protease 12 like 1 antibody
    • Ubiquitin thioesterase 12 antibody
    • Ubiquitin thiolesterase 12 antibody
    • Ubiquitin-hydrolyzing enzyme 1 antibody
    • Ubiquitin-specific-processing protease 12 antibody
    • UBP12_HUMAN antibody
    • USP 12 antibody
    • Usp12 antibody
    • USP12L1 antibody
    see all

图片

  • Anti-USP12 antibody (ab89870) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa

实验方案

文献

ab89870 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

提交于 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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