概述

  • 产品名称Anti-USP10抗体
    参阅全部 USP10 一抗
  • 描述
    兔多克隆抗体to USP10
  • 经测试应用适用于: IHC-P, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rhesus monkey, Orangutan
  • 免疫原

    A region between residue 50 and 100 of human USP10 (NP_005144.1).

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 87 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the peptidase C19 family. USP10 subfamily.
  • 翻译后修饰Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
  • 细胞定位Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 10 antibody
    • KIAA0190 antibody
    • MGC124997 antibody
    • MGC2621 antibody
    • Ubiquitin carboxyl terminal hydrolase 10 antibody
    • Ubiquitin carboxyl-terminal hydrolase 10 antibody
    • Ubiquitin specific peptidase 10 antibody
    • Ubiquitin specific processing protease 10 antibody
    • Ubiquitin specific protease 10 antibody
    • Ubiquitin thioesterase 10 antibody
    • Ubiquitin thiolesterase 10 antibody
    • Ubiquitin-specific-processing protease 10 antibody
    • UBP10_HUMAN antibody
    • UBPO antibody
    • USP 10 antibody
    • USP10 antibody
    see all

Anti-USP10 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP10 with ab70895 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-USP10 antibody (ab70895) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 87 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • Detection of Human USP10 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70895 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot detection.
  • All lanes : Anti-USP10 antibody (ab70895) at 0.1 µg/ml

    Lane 1 : TCMK-1 whole cell lysate
    Lane 2 : 4T1 whole cell lysate
    Lane 3 : CT26.WT whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 87 kDa


    Exposure time : 3 minutes
  • ICC/IF image of ab70895 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70895, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70895 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70895, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-USP10 antibody (ab70895)参考文献

This product has been referenced in:
  • Katoh H  et al. Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. J Virol 87:489-502 (2013). Read more (PubMed: 23097442) »

See 1 Publication for this product

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