Synthetic peptide, corresponding to a region within amino acids 296-346 of Human USF2 (NP_003358.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
Use at 2-10 µg/mg of lysate.
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Class B basic helix-loop-helix protein 12 antibody
Western blot - Anti-USF2 antibody (ab125184)
All lanes : Anti-USF2 antibody (ab125184) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 37 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-USF2 antibody (ab125184)
ab125184, at 6 µg/mg lysate, detecting USF2 in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125184 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.
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