Anti-Uroplakin Ib抗体(ab102961)

概述

  • 产品名称Anti-Uroplakin Ib抗体
    参阅全部 Uroplakin Ib 一抗
  • 描述
    兔多克隆抗体to Uroplakin Ib
  • 经测试应用适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 166-196 of Human Uroplakin Ib

  • 阳性对照
    • K562 cell lysate, Human Brain Tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab102961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
  • 组织特异性Bladder epithelium.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 翻译后修饰N-glycosylated with high-mannose oligosaccharides.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Tetraspanin-20 antibody
    • Tspan-20 antibody
    • TSPAN20 antibody
    • UP1b antibody
    • UPIb antibody
    • UPK1B antibody
    • UPK1B_HUMAN antibody
    • Uroplakin Ib antibody
    • Uroplakin-1b antibody
    see all

Anti-Uroplakin Ib antibody 图像

  • Anti-Uroplakin Ib antibody (ab102961) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 30 kDa
  • Immunohistochemical staining of formalin fixed and paraffin embedded brain tissue followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells (green histogram) using ab102961 compared to a negative control cell (blue histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

Anti-Uroplakin Ib antibody (ab102961)参考文献

ab102961 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (gingiva)
Specification gingiva
Permeabilization No
Fixative Formaldehyde
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提交于 Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"