Urokinase type Plasminogen Activator人Chromogenic Activity Assay试剂盒- Direct Assay (ab108916)

概述

  • 产品名称Urokinase type Plasminogen Activator人Chromogenic Activity Assay试剂盒- Direct Assay
    参阅全部 Urokinase type Plasminogen Activator 试剂盒
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型Quantitative
  • 灵敏度
    < 0.1 IU/ml
  • 种属反应性
    与反应: Human
  • 产品概述

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.

    ab108916 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.

  • 经测试应用适用于: ELISAmore details

性能

  • 功能Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 组织特异性Expressed in the prostate gland and prostate cancers.
  • 疾病相关Quebec platelet disorder
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • ATF
    • BDPLT5
    • EC 3.4.21
    • EC 3.4.21.73
    • Plasminogen Activator, Urinary
    • Plasminogen Activator, Urokinase
    • PLAU
    • QPD
    • U-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-Type Plasminogen Activator
    • Urokinase-type plasminogen activator chain B
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay 图像

  • uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)
  • uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)

实验方案

Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay (ab108916)参考文献

ab108916 has not yet been referenced specifically in any publications.

Product Wall



For uPA activity kits, we have two types of kit. One is ab108916 which is direct using uPA substrate to generate the color. The uPA substrate is specifically designed for its specificity for uPA. This assay can differentiate uPA activity vs. ...

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