Urokinase type Plasminogen活化剂人Chromogenic Activity Assay试剂盒- Direct Assay (ab108916)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma, Serum
- Sensitivity: 0.062 IU/ml
概述
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产品名称
Urokinase type Plasminogen活化剂人Chromogenic Activity Assay试剂盒- Direct Assay
参阅全部 Urokinase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Enzyme activity (quantitative) -
灵敏度
< 0.062 IU/ml -
种属反应性
与反应: Human -
产品概述
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108916 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma, serum and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 96 tests Assay Diluent 1 x 30ml Human uPA Standard 1 vial Microplate 1 unit Sealing Tapes 3 units uPA Substrate 3 units -
研究领域
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功能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
组织特异性
Expressed in the prostate gland and prostate cancers. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
细胞定位
Secreted. - Information by UniProt
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别名
- ATF
- ATF uPA
- BDPLT5
see all -
数据库链接
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108916 被引用在 1 文献中.
- Matsuyama A et al. Prognostic significance of the urokinase plasminogen activator system in tissue and serum of dogs with appendicular osteosarcoma. PLoS One 17:e0273811 (2022). PubMed: 36174075