概述

性能

应用

Our Abpromise guarantee covers the use of ab40841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 49 kDa. Approx 48kDa band observed in lysates of Human Kidney fibroblast cell line HEK293. A minor band was also consistently observed at appox 35kDa. This band was successfully blocked by incubation with the immunising peptide, and is consistent with the B chain of Cathepsin B-mediated cleavage product of PLAU (Kobayashi et al, J Biol Chem. 1991 Mar 15;266(8):5147-52; PMID: 1900515).

靶标

  • 功能
    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 组织特异性
    Expressed in the prostate gland and prostate cancers.
  • 序列相似性
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

图片

  • Anti-Urokinase antibody (ab40841) at 0.3 µg/ml + 293 lysate (35µg protein in RIPA buffer).

    Predicted band size : 49 kDa
    Observed band size : 49 kDa

实验方案

文献

This product has been referenced in:
  • Kachgal S & Putnam AJ Mesenchymal stem cells from adipose and bone marrow promote angiogenesis via distinct cytokine and protease expression mechanisms. Angiogenesis 14:47-59 (2011). WB ; Human . Read more (PubMed: 21104120) »
  • Al-Souhibani N  et al. The RNA-binding zinc-finger protein tristetraprolin regulates AU-rich mRNAs involved in breast cancer-related processes. Oncogene : (2010). WB ; Human . Read more (PubMed: 20498646) »

See all 2 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab40841.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册