概述

  • 产品名称Anti-Urokinase抗体
    参阅全部 Urokinase 一抗
  • 描述
    山羊多克隆抗体to Urokinase
  • 特异性The antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin).
  • 经测试应用适用于: Dot blot, ICC/IF, IHC-Fr, IHC-P, ELISA, WB, RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Extensively purified HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from urine (Human).

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.
  • 常规说明


    ab24122 binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. Human whole plasma or plasma proteins do not interfere with its binding to urokinase.

性能

应用

Our Abpromise guarantee covers the use of ab24122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ICC/IF Use 10µg for 10 cells.
IHC-Fr 1/50.
IHC-P 1/50.
ELISA 1/5000.
WB Use at an assay dependent concentration. Predicted molecular weight: 54, 52 , 33 kDa.
RIA Use at an assay dependent concentration.

靶标

  • 功能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 组织特异性Expressed in the prostate gland and prostate cancers.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

Anti-Urokinase antibody 图像

  • ICC/IF image of ab24122 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA /0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24122 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Urokinase antibody (ab24122)参考文献

ab24122 has not yet been referenced specifically in any publications.

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