Anti-Urocortin 3抗体(ab79121)


  • 产品名称Anti-Urocortin 3抗体
    参阅全部 Urocortin 3 一抗
  • 描述
    兔多克隆抗体to Urocortin 3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Urocortin 3.

    (Peptide available as ab90638.)

  • 阳性对照
    • This antibody gave a positive signal in human brain pituitary tissue lysate. IF/ICC: HepG2 cell line.



Our Abpromise guarantee covers the use of ab79121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • 相关性Urocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • MGC119002 antibody
    • OTTHUMP00000018999 antibody
    • Prepro urocortin 3 antibody
    • SCP antibody
    • SPC antibody
    • Stresscopin antibody
    • Ucn III antibody
    • UCN3 antibody
    • UCN3 urocortin 3 stresscopin antibody
    • UCNIII antibody
    • Urocortin 3 antibody
    • Urocortin 3 stresscopin antibody
    • Urocortin III antibody
    see all

Anti-Urocortin 3 antibody 图像

  • ICC/IF image of ab79121 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79121, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Urocortin 3 antibody (ab79121) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds

Anti-Urocortin 3 antibody (ab79121)参考文献

ab79121 has not yet been referenced specifically in any publications.

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