Anti-URB2抗体(ab121292)

概述

  • 产品名称Anti-URB2抗体
    参阅全部 URB2 一抗
  • 描述
    兔多克隆抗体to URB2
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 1066-1205 of Human URB2.

  • 阳性对照
    • Human kidney tissue; U 2 OS cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C77575 antibody
    • KIAA0133 antibody
    • mKIAA0133 antibody
    • NET10 antibody
    • NPA2 antibody
    • nucleolar preribosomal-associated protein 1 antibody
    • RGD1307980 antibody
    • Unhealthy ribosome biogenesis protein 2 homolog antibody
    • URB2 antibody
    • URB2 ribosome biogenesis 2 homolog (S. cerevisiae) antibody
    • URB2_HUMAN antibody
    see all

Anti-URB2 antibody 图像

  • ab121292 at 1/75 dilution staining URB2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab121292 at 4 µg/ml staining URB2 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
  • All lanes : Anti-URB2 antibody (ab121292) at 1/1000 dilution

    Lane 1 : Hela extracts 25ug
    Lane 2 : RPE-1 extracts 25ug
    Lane 3 : Hela extracts 50ug
    Lane 4 : RPE-1 extracts 50ug

    Secondary
    Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa (possible cross reactivity).

    Exposure time : 3 minutes

    See Abreview

Anti-URB2 antibody (ab121292)参考文献

ab121292 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount 25 µg
Specification HeLa and RPE-1
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"