antigen sequence, corresponding to amino acids 1066-1205 of Human URB2.
- Human kidney tissue; U 2 OS cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Nucleus > nucleolus.
Information by UniProt
- C77575 antibody
- KIAA0133 antibody
- mKIAA0133 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URB2 antibody (ab121292)
ab121292 at 1/75 dilution staining URB2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-URB2 antibody (ab121292)
ab121292 at 4 µg/ml staining URB2 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
Western blot - Anti-URB2 antibody (ab121292)
All lanes :
Anti-URB2 antibody (ab121292) at 1/1000 dilutionLane 1 :
Hela extracts 25ugLane 2 :
RPE-1 extracts 25ugLane 3 :
Hela extracts 50ugLane 4 :
RPE-1 extracts 50ugSecondary
Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution
Performed under reducing conditions.Observed band size :
130 kDa (why is the actual band size different from the predicted?
)Additional bands at :
39 kDa (possible cross reactivity).Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"