重组Anti-UQCRH抗体[EPR9038(B)] (ab154803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9038(B)] to UQCRH
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-UQCRH抗体[EPR9038(B)]
参阅全部 UQCRH 一抗 -
描述
兔单克隆抗体[EPR9038(B)] to UQCRH -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal liver, PC12, C6, fetal brain, Human spleen and Saos 2 lysates; Human colon and liver tissues; Saos 2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9038(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154803于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c. -
细胞定位
Mitochondrial. Mitochondrion inner membrane. -
数据库链接
- Entrez Gene: 7388 Human
- Entrez Gene: 66576 Mouse
- Entrez Gene: 366448 Rat
- Omim: 613844 Human
- SwissProt: P07919 Human
- SwissProt: P99028 Mouse
- SwissProt: Q5M9I5 Rat
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别名
- Complex III subunit 6 antibody
- Complex III subunit VIII antibody
- Cytochrome b c1 complex subunit 6 antibody
see all
图片
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All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : PC12 lysate
Lane 3 : C6 lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human spleen lysate
Lane 6 : Saos 2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).
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Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154803 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab154803 被引用在 2 文献中.
- Yu H et al. Platelet biomarkers for a descending cognitive function: A proteomic approach. Aging Cell 20:e13358 (2021). PubMed: 33942972
- Meng S et al. The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis. Proteome Sci 17:8 (2019). PubMed: 31889914