概述

  • 产品名称
  • 描述
    兔多克隆抗体to UQCRC2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length Human UQCRC2 (NP_003357.2, amino acids 1-453).

  • 阳性对照
    • Human colon and Mouse kidney tissue lysates; A431 cell lysate; UQCRC2 transfected 293T cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

应用

Our Abpromise guarantee covers the use of ab103616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • 序列相似性
    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

图片

  • ICC/IF image of ab103616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103616 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 48 kDa
  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size : 48 kDa
  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 48 kDa
  • All lanes : Anti-UQCRC2 antibody (ab103616) at 5 µg/ml

    Lane 1 : UQCRC2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 48 kDa

文献

This product has been referenced in:
  • Basisty N  et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy? Aging Cell 15:634-45 (2016). WB ; Mouse . Read more (PubMed: 27061426) »
  • Chong UR  et al. Effects of Tamarindus indica fruit pulp extract on abundance of HepG2 cell lysate proteins and their possible consequential impact on metabolism and inflammation. Biomed Res Int 2013:459017 (2013). Read more (PubMed: 24455694) »

See all 2 Publications for this product

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