概述

  • 产品名称Anti-UPF3A抗体
    参阅全部 UPF3A 一抗
  • 描述
    小鼠单克隆抗体to UPF3A
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: TGGGKQESCA PGAVVKARPM EGSLEEPQET SHSGSDKEHR DVERSQEQES EAQRYHVDDG RRHRAHHEPE RLSRRSEDEQ RWGKGPGQDR GKKGSQDSGA P, corresponding to amino acids 274-375 of Human UPF3A (AAH23569) with tag.

  • 阳性对照
    • UPF3A transfected 293T cell lysate; Recombinant tagged human UPF3A fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Part of a multiprotein post-splicing mRNP complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA upstream of exon-exon junctions.
  • 组织特异性Isoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord.
  • 序列相似性Belongs to the RENT3 family.
  • 细胞定位Nucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2600001C03Rik antibody
    • 4930546M19Rik antibody
    • hUpf3 antibody
    • HUPF3A antibody
    • MGC151365 antibody
    • Nonsense mRNA reducing factor 3A antibody
    • OTTHUMP00000018789 antibody
    • Regulator of nonsense transcripts 3A antibody
    • REN3A_HUMAN antibody
    • RENT3A antibody
    • Up frameshift suppressor 3 homolog A antibody
    • Up-frameshift suppressor 3 homolog A antibody
    • UPF3 A antibody
    • UPF3 antibody
    • UPF3 regulator of nonsense transcripts homolog A antibody
    • upf3a antibody
    see all

Anti-UPF3A antibody 图像

  • Anti-UPF3A antibody (ab77423) at 1 µg/ml + Recombinant tagged human UPF3A fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-UPF3A antibody (ab77423) at 1 µg/ml

    Lane 1 : UPF3A transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-UPF3A antibody (ab77423)参考文献

ab77423 has not yet been referenced specifically in any publications.

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