Anti-uPA Receptor/U-PAR抗体(ab103791)
Key features and details
- Rabbit polyclonal to uPA Receptor/U-PAR
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-uPA Receptor/U-PAR抗体
参阅全部 uPA Receptor/U-PAR 一抗 -
描述
兔多克隆抗体to uPA Receptor/U-PAR -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab103791于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
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ICC |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. |
ICC
Use a concentration of 1 µg/ml. |
靶标
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功能
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
组织特异性
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
序列相似性
Contains 3 UPAR/Ly6 domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5329 Human
- Entrez Gene: 50692 Rat
- Omim: 173391 Human
- SwissProt: Q03405 Human
- SwissProt: P49616 Rat
- Unigene: 466871 Human
- Unigene: 82711 Rat
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别名
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
see all
图片
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All lanes : Anti-uPA Receptor/U-PAR antibody (ab103791) at 1 µg/ml
Lane 1 : Human placenta tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : MCF7 whole cell lysate
Lane 6 : SMMC whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : Raji whole cell lysate
Lane 9 : Colo320 whole cell lysate
Predicted band size: 37 kDa -
ab103791, at 1µg/ml, staining uPA Receptor/U-PAR in MCF7 cells by Immunocytochemistry.
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MDA-MB-231 cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor/U-PAR in MDA-MB-231 cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab103791 被引用在 10 文献中.
- Fu X et al. Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. Liver Int 42:696-709 (2022). PubMed: 34854209
- Abufaraj M et al. The expression of urokinase-type plasminogen activator system in upper tract urothelial carcinoma and its prognostic value after radical nephroureterectomy. Urol Oncol N/A:N/A (2020). PubMed: 32381392
- Gu C et al. Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1. Oncol Res 27:515-524 (2019). PubMed: 29386086
- Cammalleri M et al. The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model. J Cell Mol Med 23:5176-5192 (2019). PubMed: 31251468
- Wach S et al. Exploring the MIR143-UPAR Axis for the Inhibition of Human Prostate Cancer Cells In Vitro and In Vivo. Mol Ther Nucleic Acids 16:272-283 (2019). PubMed: 30933831
- Huang JM et al. Ulinastatin Inhibits Osteoclastogenesis and Suppresses Ovariectomy-Induced Bone Loss by Downregulating uPAR. Front Pharmacol 9:1016 (2018). PubMed: 30245631
- Shang X et al. Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding. Cell Mol Life Sci N/A:N/A (2018). PubMed: 30032357
- Hao XD et al. De novo mutations of TUBA3D are associated with keratoconus. Sci Rep 7:13570 (2017). PubMed: 29051577
- Puthusseri B et al. Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 312:L783-L796 (2017). PubMed: 28385810
- Al Dhaheri Y et al. Anti-metastatic and anti-tumor growth effects of Origanum majorana on highly metastatic human breast cancer cells: inhibition of NF?B signaling and reduction of nitric oxide production. PLoS One 8:e68808 (2013). WB . PubMed: 23874773