概述

  • 产品名称Anti-UNG抗体
    参阅全部 UNG 一抗
  • 描述
    小鼠多克隆抗体to UNG
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human UNG protein (NP_003353.1).

  • 阳性对照
    • Human placenta tissue lysate. Cell lysate from transfected 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab89640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • 组织特异性Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • 疾病相关Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • 序列相似性Belongs to the uracil-DNA glycosylase family.
  • 翻译后修饰Isoform 1 is processed by cleavage of a transit peptide.
  • 细胞定位Mitochondrion and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Anti-UNG antibody 图像

  • Anti-UNG antibody (ab89640) at 1 µg/ml + human placenta tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : ~30 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-UNG antibody (ab89640) at 1 µg/ml

    Lane 1 : cell lysate from UNG transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : ~30 kDa. We are unsure as to the identity of these extra bands.
  • ab89640 at 10 µg/ml staining UNG in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

Anti-UNG antibody (ab89640)参考文献

ab89640 has not yet been referenced specifically in any publications.

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