概述

  • 产品名称Anti-UNC5D抗体
  • 描述
    小鼠单克隆抗体to UNC5D
  • 经测试应用适用于: WB, ICC/IF, Flow Cyt, IHC - Wholemountmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
  • 免疫原

    Recombinant fragment: TDNGEALPES IPSAPGTLPH FIEEPDDAYI IKSNPIALRC KARPAMQIFF KCNGEWVHQN EHVSEETLDE SSGLKVREVF INVTRQQVED FHGPEDYWCQ , corresponding to amino acids 31-131 of Human UNC5D

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC - Wholemount Use at an assay dependent concentration.

靶标

  • 相关性UNC5D belongs to a family of netrin 1 receptors thought to mediate the chemorepulsive effect of netrin 1 on specific axons. On ligand binding UNC5D may mediate axon repulsion of neuronal growth cones in the developing nervous system.
  • 细胞定位Membrane; Single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • FLJ16019 antibody
    • KIAA1777 antibody
    • Netrin receptor UNC5D antibody
    • Netrin receptor Unc5h4 antibody
    • PRO34692 antibody
    • Protein unc 5 homolog D antibody
    • Unc 5 homolog 4 antibody
    • Unc 5 homolog D (C. elegans) antibody
    • Unc 5 homolog D antibody
    • UNC 5D antibody
    • Unc5h4 antibody
    see all

Anti-UNC5D antibody 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab58141 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58141, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Western blot against tagged recombinant protein immunogen using ab58141 UNC5D antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab58141 staining UNC5D in chicken whole mount brain by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 10% serum for 48 hours. Samples were then incubated with primary antibody at 1/500 for 48 hours. The secondary antibody used was a goat anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

Anti-UNC5D antibody (ab58141)参考文献

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer, pH 6
Sample Mouse Tissue sections (E17 mouse brain neocortex)
Specification E17 mouse brain neocortex
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Bhavin Shah

Verified customer

提交于 Jul 17 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (Embryonic brain (cortex), E15.5)
Specification Embryonic brain (cortex), E15.5
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 11 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (Mouse Cortical neurons, E14.5)
Specification Mouse Cortical neurons, E14.5
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 11 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step None
Sample Ferret Tissue sections (Forebrain)
Specification Forebrain
Permeabilization Yes - 0.3% Triton-X
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

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Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (whole-mount brain)
Specification whole-mount brain
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Oct 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"