概述

  • 产品名称Anti-UNC5C抗体
    参阅全部 UNC5C 一抗
  • 描述
    兔多克隆抗体to UNC5C
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the center region of Human UNC5C conjugated to KLH (NP_003719.2)

  • 阳性对照
    • K562 cell lysate# This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

性能

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应用

Our Abpromise guarantee covers the use of ab91408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 103 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
    • 组织特异性Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.
    • 序列相似性Belongs to the unc-5 family.
      Contains 1 death domain.
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 2 TSP type-1 domains.
      Contains 1 ZU5 domain.
    • 翻译后修饰Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.
      Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • homolog of C. elegans transmembrane receptor Unc5 antibody
      • Netrin receptor UNC5C antibody
      • Protein unc 5 homolog C antibody
      • Protein unc-5 homolog 3 antibody
      • Protein unc-5 homolog C antibody
      • Unc 5 homolog 3 antibody
      • Unc 5 homolog C antibody
      • Unc5 (C.elegans homolog) c antibody
      • Unc5c antibody
      • UNC5C_HUMAN antibody
      • UNC5H3 antibody
      see all

    Anti-UNC5C antibody 图像

    • ICC/IF image of ab91408 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91408 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-UNC5C antibody (ab91408) at 1/100 dilution + K562 cell lysate at 35 µg

      Predicted band size : 103 kDa

    Anti-UNC5C antibody (ab91408)参考文献

    ab91408 has not yet been referenced specifically in any publications.

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