概述

  • 产品名称
    Anti-UNC5B抗体[Guido-1]
    参阅全部 UNC5B 一抗
  • 描述
    小鼠单克隆抗体[Guido-1] to UNC5B
  • 特异性
    ab54430 recognizes UNC5B.
  • 经测试应用
    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 28-363 of Human UNC5B

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab54430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
  • 组织特异性
    Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
  • 序列相似性
    Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 TSP type-1 domains.
    Contains 1 ZU5 domain.
  • 翻译后修饰
    Phosphorylated on cytoplasmic tyrosine residues.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
    Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.
  • 细胞定位
    Cell membrane. Membrane raft. Associated with lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6330415E02Rik antibody
    • A630020F16 antibody
    • D10Bwg0792e antibody
    • Netrin receptor UNC5B antibody
    • Netrin receptor UNC5B Precursor antibody
    • p53 regulated receptor for death and life antibody
    • p53 regulated receptor for death and life protein 1 antibody
    • p53-regulated receptor for death and life protein 1 antibody
    • p53RDL1 antibody
    • Protein unc-5 homolog 2 antibody
    • Protein unc-5 homolog B antibody
    • Protein XUNC 5 antibody
    • Transmembrane receptor Unc5H2 antibody
    • Unc 5 homolog 2 antibody
    • Unc 5 homolog B antibody
    • Unc 5 netrin receptor B antibody
    • UNC 5B antibody
    • Unc5 (C.elegans homolog) b antibody
    • Unc5b antibody
    • UNC5B_HUMAN antibody
    • UNC5H2 antibody
    see all

图片

  • ab54430 staining UNC5B in mouse erythrocytes by Flow Cytometry. Collected heparinised mouse blood was incubated with erythrocyte lysing solution for 5 minutes at room temperature. The cells were washed and incubated with the primary antibody, CD45 as a general marker for leukocytes (green) and F4/80 to label macrophages (blue).

  • ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Miloudi K  et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). Read more (PubMed: 27400127) »
  • Ramkhelawon B  et al. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med 20:377-84 (2014). IF . Read more (PubMed: 24584118) »

See all 3 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Whole skin 40 µm sections)
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100 (0.3%)
Specification
Whole skin 40 µm sections
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Mr. Thomas Klein

Verified customer

提交于 Aug 26 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (normal tissue array)
Specification
normal tissue array
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
serum free protein blocker as blocking agent for 30 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 Apr 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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