Anti-Unc5a抗体(ab81165)

概述

  • 产品名称Anti-Unc5a抗体
    参阅全部 Unc5a 一抗
  • 描述
    兔多克隆抗体to Unc5a
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a section of internal sequence amino acids 323-372 of Unc5a: ILVYCRKKEG LDSDVADSSI LTSGFQPVSI KPSKADNPHL LTIQPDLSTT

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab81165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • 序列相似性Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 TSP type-1 domain.
    Contains 1 ZU5 domain.
  • 结构域The ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.
  • 翻译后修饰Phosphorylated on cytoplasmic tyrosine residues. Phosphorylated by PKC in vitro.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • 细胞定位Cell membrane. The interaction with PRKCABP regulates its surface expression and leads to its removal from surface of neurons and growth cones.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ16449 antibody
    • KIAA1976 antibody
    • Netrin receptor UNC 5A antibody
    • Netrin receptor UNC5A antibody
    • Netrin receptor Unc5h1 antibody
    • Protein unc-5 homolog 1 antibody
    • Protein unc-5 homolog A antibody
    • Unc 5 homolog 1 antibody
    • Unc 5 homolog A antibody
    • Unc 5a antibody
    • Unc5 a antibody
    • Unc5 homolog 1 antibody
    • Unc5 homolog A antibody
    • Unc5a antibody
    • UNC5A_HUMAN antibody
    • Unc5h1 antibody
    see all

Anti-Unc5a antibody 图像

  • Anti-Unc5a antibody (ab81165) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    Use at 1/100000

    Predicted band size : 93 kDa

Anti-Unc5a antibody (ab81165)参考文献

ab81165 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0.25%TritonX100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 13 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Sample African Green Monkey Cell lysate - whole cell (COS cells transfected with human Unc5)
Specification COS cells transfected with human Unc5
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Nov 04 2014

The observed MW for this antibody is in fact lower than the predicted MW. Generally a lower observed MW is the results form isoforms produced from splice variants. There are in fact 4 predicted isoforms for the UNC5 target and most likely the observed...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"