概述

  • 产品名称Anti-umuD抗体
  • 描述
    兔多克隆抗体to umuD
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Purified recombinant LacZ-UmuD fusion protein

  • 阳性对照
    • Protein extract from E. coli

性能

应用

Our Abpromise guarantee covers the use of ab91343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/3000. Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性UmuD is a DNA polymerase V subunit capable of translesion synthesis and inducible mutagenesis, by radiation or chemical agents. Transcription of umuD is repressed by a repressor, LexA protein in uninduced cells. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as a error-prone translesion DNA polymerase. The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14KD.
    • 数据库链接
      • 别名
        • DNA polymerase V subunit D antibody
        • ECK1171 antibody
        • JW1172 antibody
        • Protein umuD antibody

      Anti-umuD antibody 图像

      • All lanes : Anti-umuD antibody (ab91343) at 1/3000 dilution

        Lane 1 : protein extracts from E. coli DE274 (lexA51, recA730)
        Lane 2 : protein extract from E. coli DE274 (lexA51, recA730) treated with mitomycin C


        Predicted band size : 14 kDa

      Anti-umuD antibody (ab91343)参考文献

      This product has been referenced in:
      • Naiman K  et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A 111:5526-31 (2014). Read more (PubMed: 24706928) »
      • Cafarelli TM  et al. A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli. J Bacteriol 195:1179-93 (2013). Escherichia coli . Read more (PubMed: 23292773) »

      See all 2 Publications for this product

      Product Wall

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Western blot
      Sample Escherichia coli Cell lysate - whole cell (bacteria)
      Loading amount 1e+009 cells
      Specification bacteria
      Gel Running Conditions Non-reduced Denaturing (15%)
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Username

      Abcam user community

      Verified customer

      提交于 Feb 12 2013

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"