The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/3000. Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
UmuD is a DNA polymerase V subunit capable of translesion synthesis and inducible mutagenesis, by radiation or chemical agents. Transcription of umuD is repressed by a repressor, LexA protein in uninduced cells. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as a error-prone translesion DNA polymerase.
The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14KD.
Naiman K et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A111:5526-31 (2014).
Read more (PubMed: 24706928) »
Cafarelli TM et al. A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli. J Bacteriol195:1179-93 (2013).
Read more (PubMed: 23292773) »