概述

  • 产品名称Anti-UMPS抗体
    参阅全部 UMPS 一抗
  • 描述
    兔多克隆抗体to UMPS
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide: FVVGFISGSR VSMKPEFLHL TPGVQLEAGG DNLGQQYNSP QEVIGKRGSD , corresponding to C terminal amino acids 395-444 of Human UMPS

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab80857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

靶标

  • 通路Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
  • 疾病相关Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.
  • 序列相似性In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
    In the C-terminal section; belongs to the OMP decarboxylase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ODC antibody
    • OMPdecase antibody
    • OPRT antibody
    • OPRTase antibody
    • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase antibody
    • Orotate phosphoribosyltransferase antibody
    • Orotate phosphoribosyltransferase phosphoribosyltransferase antibody
    • Orotidine 5' phosphate decarboxylase antibody
    • Orotidine 5''-phosphate decarboxylase antibody
    • RP11-71H17.9 antibody
    • UMP synthase antibody
    • Umps antibody
    • UMPS_HUMAN antibody
    • Uridine 5' monophosphate synthase antibody
    • Uridine monophosphate synthetase antibody
    see all

Anti-UMPS antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling UMPS with ab80857 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - UMPS. Right - Merge.
  • Anti-UMPS antibody (ab80857) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Gel concentration 12%

Anti-UMPS antibody (ab80857)参考文献

ab80857 has not yet been referenced specifically in any publications.

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