概述

  • 产品名称Anti-ULK3抗体[EPR4888]
    参阅全部 ULK3 一抗
  • 描述
    兔单克隆抗体[EPR4888] to ULK3
  • 经测试应用适用于: WB, ICC, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human ULK3.

  • 阳性对照
    • IHC-P: Human bladder carcinoma tissue WB: Human heart, testis, fetal kidney, LnCaP and 293T cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
ICC 1/25 - 1/50.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
    • 组织特异性Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 2 MIT domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated in vitro.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 形式There are 3 isoforms produced by alternative splicing.
    • 别名
      • DKFZP434C131 antibody
      • FLJ90566 antibody
      • Serine/threonine-protein kinase ULK3 antibody
      • Ulk3 antibody
      • ULK3_HUMAN antibody
      • unc 51 like kinase 3 (C. elegans) antibody
      • Unc-51-like kinase 3 antibody
      see all

    Anti-ULK3 antibody [EPR4888] 图像

    • All lanes : Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human testis lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : LnCaP cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Anti-ULK3 antibody [EPR4888] (ab124947)参考文献

    This product has been referenced in:
    • Hutterer C  et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . Read more (PubMed: 23648710) »

    See 1 Publication for this product

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