概述

  • 产品名称Anti-ULK3抗体
    参阅全部 ULK3 一抗
  • 描述
    兔多克隆抗体to ULK3
  • 特异性Detects endogenous levels of total ULK3 protein.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of Human ULK3, isoform 2.

  • 阳性对照
    • Human breast carcinoma tissue; 293 cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.88% Sodium chloride, 49.1% PBS
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111195 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
  • 组织特异性Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 2 MIT domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated in vitro.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式There are 3 isoforms produced by alternative splicing.
  • 别名
    • DKFZP434C131 antibody
    • FLJ90566 antibody
    • Serine/threonine-protein kinase ULK3 antibody
    • Ulk3 antibody
    • ULK3_HUMAN antibody
    • unc 51 like kinase 3 (C. elegans) antibody
    • Unc-51-like kinase 3 antibody
    see all

Anti-ULK3 antibody 图像

  • ab111195 at 1/50 dilution staining ULK3 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.
  • All lanes : Anti-ULK3 antibody (ab111195) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa

Anti-ULK3 antibody (ab111195)参考文献

ab111195 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111195.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"