概述

  • 产品名称
  • 描述
    兔多克隆抗体to ULK2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 693 and 938 of Human ULK2 (NP_055498).

  • 阳性对照
    • 293T, H1299, HeLa, Molt-4, Raji cell lysates. HepG2 whole cell lysates. HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG1B antibody
    • KIAA0623 antibody
    • Serine/threonine protein kinase ULK2 antibody
    • Serine/threonine-protein kinase ULK2 antibody
    • ULK2 antibody
    • ULK2_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 2 antibody
    • Unc 51 like autophagy activating kinase 2 antibody
    • Unc 51 like kinase 2 antibody
    • Unc-51-like kinase 2 antibody
    • Unc51.2 antibody
    see all

图片

  • Anti-ULK2 antibody (ab97695) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 113 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97695 at 1/200 dilution. Lower image is merged with DNA probe.

文献

This product has been referenced in:
  • Puto LA  et al. Transcriptional Repressor DAXX Promotes Prostate Cancer Tumorigenicity via Suppression of Autophagy. J Biol Chem 290:15406-20 (2015). WB ; Human . Read more (PubMed: 25903140) »
  • Konno H  et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). Read more (PubMed: 24119841) »

See all 2 Publications for this product

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2017

Thank you for your reply, and for your patience in resolving this issue. I have asked that these three replacement vials be added to your next shipment. Please let me know if you have any questions or if there is anything else that we can do for you.

I have processed your request to receive ab56736 as a replacement for ab97695. You r new order number is ******.

I am sorry that the protocol tips did not prove to be successful and I would be happy to send you ab56736 as a replacement. I would just like to confirm the original order information. Can you confirm that it was ordered by yourself on*******. I just l...

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Thank you for your reply. I would be happy to send replacements. Does the customer wish to try fresh vials of the antibodies they have already tried, or would they prefer different antibodies to the targets NPTX2, NUCKS1, and ULK2? I look forward to he...

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Thank you for contacting us and for confirming the details of your experiment. I am sorry that this antibody did not perform as stated on the datasheet and your protocol seems fine. This amount will be refunded to the credit card used to make this ...

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Thank you for your reply. I would be happy to offer the customer a free of charge replacement or credit. Please let me know how the customer would like to proceed and if they would like a replacement, which product they would like to receive. I would b...

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Thanks for your reply and for sending the additional information. I do have a couple of additional suggestions, however I am not sure if the customer has tried these steps already. For the antibodies ab97695, ab69858, and ab7770, we would recomm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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