概述

  • 产品名称Anti-ULK1抗体
    参阅全部 ULK1 一抗
  • 描述
    小鼠单克隆抗体to ULK1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 602-716 of Human ULK1

性能

应用

Our Abpromise guarantee covers the use of ab56344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
    • 组织特异性Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
    • 细胞定位Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATG 1 antibody
      • ATG1 antibody
      • ATG1 autophagy related 1 homolog antibody
      • ATG1A antibody
      • Autophagy related protein 1 homolog antibody
      • Autophagy-related protein 1 homolog antibody
      • FLJ38455 antibody
      • FLJ46475 antibody
      • hATG1 antibody
      • KIAA0722 antibody
      • Serine/threonine protein kinase ULK1 antibody
      • Serine/threonine protein kinase Unc51.1 antibody
      • Serine/threonine-protein kinase ULK1 antibody
      • ULK 1 antibody
      • ULK1 antibody
      • ULK1_HUMAN antibody
      • Unc 51 (C. elegans) like kinase 1 antibody
      • UNC 51 antibody
      • Unc 51 like kinase 1 antibody
      • Unc-51 like kinase 1 (C. elegans) antibody
      • Unc-51-like kinase 1 antibody
      • UNC51 antibody
      • UNC51, C. elegans, homolog of antibody
      • Unc51.1 antibody
      see all

    Anti-ULK1 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56344 ULK1 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa

    Anti-ULK1 antibody (ab56344)参考文献

    ab56344 has not yet been referenced specifically in any publications.

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